A new functional assay of thrombin activatable fibrinolysis inhibitor
作者： A. H. C. GUIMARÃES , R. M. BERTINA , D. C. RIJKEN
DOI: 10.1111/J.1538-7836.2005.01388.X
关键词:
摘要: Summary. New thrombin activatable fibrinolysis inhibitor (TAFI) assays are necessary for studying the role of this in cardiovascular disease. The identification a functional single nucleotide polymorphism (SNP) (1040C/T) leading to TAFI-variant with increased stability but lower antigen levels has made determination activity even more essential. Therefore, we developed new assay TAFI citrated plasma samples. This is based on retardation clot lysis by TAFIa. activation was induced simultaneously fibrin formation and mediated rt-PA. variability other components minimized 20-fold dilution samples TAFI-depleted plasma. Lysis times (−/+ potato carboxypeptidase inhibitor) TAFI-related lysis, parameter assay, were determined group 92 healthy volunteers, as well (electroimmunoassay) two SNPs (−438A/G 1040C/T). 19.8 ± 5.6 min (mean ± SD) correlated level. specific antifibrinolytic associated −438A/G 1040C/T genotypes. Individuals 325Ile-variant had average 34% higher TAFI-specific than individuals 325Thr-isoform. groups did not differ, level compensated 325Ile-isoform. provides valuable information about performance different isoforms constitutes method
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