Endoglin is expressed on a subpopulation of immature erythroid cells of normal human bone marrow.

摘要: A monoclonal antibody (1G2) was raised by immunization of a Balb/c mouse with the leukemic blasts from patient suffering chronic myelogenous leukemia blast crisis (CML-BC). Sequential immunoprecipitation protein human umbilical vein endothelial cells 1G2 and endoglin-specific 44G4 indicated that both antibodies react same molecule, homodimer molecular mass 180,000. This first identified on acute lymphoblastic shown to be primarily associated cells. In addition, subpopulation bone marrow mononuclear (BMMNC), as established two colour immunofluorescence analysis. By cell sorting colony assays it could demonstrated endoglin is not expressed hemopoietic precursor (CFU-G, CFU-GM, CFU-GEMM, BFU-E). May-Grunwald-Giemsa staining sorted BMMNC revealed recognized immature proerythroblasts double-fluorescence analysis showed present subset glycophorin A-positive BMMNC. reactive B-cells (CD19, CD20), T-cells (CD3, CD7), natural killer (CD56), myeloid (CD13, CD14, CD15, CD33), CD34-positive Endoglin contains an arginine-glycine-aspartic acid sequence, feature generally extracellular matrix proteins which interact integrins. It suggested may utilize integrins in cell-cell adhesion events stromal or compartment marrow.