In vitro effect of artemisinin on microbial biomasses, enzyme activities and composition of bacterial community

摘要: Abstract The anti-malarial drug artemisinin is extracted from the leaves of Artemisia annua L. potential risk released leaf debris and root exudation this medicinal plant unknown for microbes in soils where commercial cultivation A. practiced. Thus, a soil incubation experiment was conducted to compare microbial biomass, enzyme activities, bacterial community compositions by different concentrations (without artemisinin, CK; 10 mg kg－1 soil, LA; 20 mg kg－1 HA). results showed that compound decreased significantly biomass carbon nitrogen, activities dehydrogenases urease, implying inhibition on growth, reproduction, metabolism some bacteria, taking into account facts includes those bacteria cannot act their own without viable hosts. There were 13064, 11738, 10107 16S rDNA sequences picked up CK, LA, HA, respectively, MiSeq Illumina sequencing, which attributed 541 (CK), 453 (LA), 414 phylotypes (HA). Soils received lower richness diversity indexes than CK. Less group number present could encourage over reproduction any single taxon thus increase disease occurrence. All studied dominated three phyla (Acidobacteria, Proteobacteria, Actinobacteria), with these accounting 60.32-70.43% total bacteria. However, most predominant varied abundances between CK treatment. Among top 20 five seven unique each studied. Therefore, might exhibit selective antibacterial action towards cause great changes structure, influence, at least part, processes functions.