New Nuclease from Extremely Psychrophilic Microorganism Psychromonas ingrahamii 37: Identification and Characterization.

摘要: Nucleases are an important group of hydrolases that degrade nucleic acids, with broad spectrum applications in science and industry. In this paper, we report the identification characterization nuclease from extremely psychrophilic bacterium Psychromonas ingrahamii grows exponentially at 5 °C, but may also grow even lower temperatures (down to − 12 °C). The putative endonuclease I gene, identified genome P. ingrahamii, was cloned expressed Pichia pastoris. recombinant protein purified its nucleolytic features were studied. new enzyme, named by us as PinNuc, displays characteristic for nonselective endonucleases, has ability different forms acids. It is very active room temperature low ion-strength buffer presence concentrations magnesium ions. which possesses six cysteine residues, most likely all engaged disulphide bridges, only oxidized form, can be efficiently inactivated addition amounts a reducing agent. According our knowledge, it first nuclease, belonging family, isolated such organism.