Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes.

摘要: Abstract Molecular clones of human immunodeficiency virus type 1 that contained either 37 point mutations in the Rev-responsive element (RRE) did not affect overlapping env reading frame or both a mutated RRE and two eliminated Rev were constructed. The shown to remove negative Rev-inducible positive effects on gene expression (G. Nasioulas, A. S. Zolotukhin, C. Tabernero, L. Solomin, P. Cunningham, G. N. Pavlakis, B. K. Felber, J. Virol. 68:2986-2993, 1994). Upon insertion cis-acting simian retrovirus (SRV-1) into these clones, RRE(-) Rev(-)RRE(-) expressed efficiently. SRV-1 has properties similar those recently identified Mason-Pfizer monkey (M. Bray, Prasad, W. Dubay, E. Hunter, K.-T. Jeang, D. Rekosh, M.-L. Hammarskjold, Proc. Natl. Acad. Sci. USA 4:1256-1260, We demonstrated preparations produced after transfections element-containing molecular cells infectious cell-free transmission, they replicated about 5 10 times less efficiently than wild-type virus, propagated continuously for more 7 months peripheral blood mononuclear cells. Growth characteristics sequence analysis viruses long-term culture no RRE(+)Rev(+) revertants developed. These data demonstrate can be replaced by heterologous regulatory systems, resulting efficient production. prepared tissue used further studies life cycle virus. also suggest acts exclusively through interaction it does have any additional essential function