Bio-refinery of waste streams for green and efficient succinic acid production by engineered Yarrowia lipolytica without pH control

摘要: Abstract This paper presents an efficient, green and sustainable approach for succinic acid (SA) fermentation, in which glucose-rich hydrolysates from various waste streams were used as the substrate SA production by Yarrowia lipolytica without pH control first time. First, Y. PGC202 was found to be able consume mixed food (MFW) hydrolysate with high efficiency. After medium optimization, a titer at 31.7 g/L yield of 0.52 g/g productivity 0.60 g/L/h obtained isFBB fermentation when using 60 g/L glucose-containing MFW carbon source supplemented 3% tryptone. When other used, highest 0.61 g/g via yeast low achieved, demonstrated extensive adaptability great potential lipolytica. By feeding concentrated hydrolysate, up 71.6 g/L achieved fed-batch decreased naturally 2.8. Finally, revised recovery method, 68.3% total purity between 89.5% 91.0% recovered acidic broth only input acidification, advantages over that under neutral conditions terms economic improvement environmental sustainability. In general, this study overcomes one major drawbacks future feasible large-scale fermentative production, it also illustrates promising prospective bio-SA production.