Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriation.
作者： D.L. Knook , E.Ch. Sleyster
DOI: 10.1016/0014-4827(76)90605-4
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摘要: Abstract Isolated non-parenchymal cells from rat liver were separated by centrifugal elutriation into two fractions consisting of structurally intact Kupffer and endothelial with purities 91 95%, respectively. Purified showed nearly equal specific activities for the lysosomal enzyme acid phosphatase, whereas activity cathepsin D was about 3 times higher in cells. It calculated that a significant amount is present
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