Effect of Alloxan on Insulin Secretion in Isolated Rat Islets Perifused in Vitro

摘要: Isolated rat islets were maintained in vitro a simple perifusion system and the rate of insulin secretion was throughout period perifusion. Exposure perifused to alloxan (20 mg. per cent) for five minutes produced complete inhibition subsequent glucose-induced with retention basal secretion. Glucose, mannose 3-0 methyl glucose provided or almost protection from inhibitory effect on release. The order potencies various hexoses protect against toxicity (100 ⋝ 3−0 > 2-deoxyglucose galactose fructose ≫ L-glucose (0). Mannoheptulose diminished but did not abolish protective alloxan. first phase tolbutamide-induced retained after exposure This finding indicated that beta cells still viable suggests separate receptor transport site tolbutamide may exist cell. possible mechanisms by which are discussed.