Lectin-binding analysis in biofilm systems.

摘要: Publisher Summary This chapter discusses the lectin-binding analysis in biofilm systems. The difficulty of isolating a single polymer type from complex matrix may be comparable to situation at cellular level. As consequence, there is need establish an situ technique for assessment glycoconjugate distribution At present, most promising approach achieve this application analyses combination with confocal laser scanning microscopy (CLSM). applicability lectins probe living and fully hydrated systems based on definition biofilms. A general biofilms collection microorganisms their extracellular polymeric substances (EPS) associated interface. Extracellular are defined as organic polymers biological origin, which responsible interaction interfaces. EPS include polysaccharides, proteins, nucleic acids, amphiphilic compounds. Thus, systems, one can expect two types carbohydrate structures: (1) those located cell surfaces (2) extracellularly throughout matrix.