Simple Protocol for Distinguishing Drug-induced Effects on Spatial Memory Acquisition, Consolidation and Retrieval in Mice Using the Morris Water Maze

摘要: The Morris water maze (MWM) is one of the most commonly used tests for assessing spatial learning and memory in mice. While MWM highly amenable to testing effects modifying drugs, studies do not consider timing or duration drug exposure when conducting assay; factors that can strongly influence effect on different stages interfere with data interpretation. Herein we describe a protocol which offers advantage distinguishing impact fast acting intraperitoneally (IP) injected memory: acquisition, consolidation, retrieval. Mice initially undergo habituation both apparatus IP injection procedure over course three days. For mice are prior training sessions four consecutive days, where learn find an escape platform circular tank using distal cues. To determine immediately after each session. retrieval, receive mock injections day only once, probe trial, attempt locate following its removal from tank. This provides simple strategy effect(s) CNS memory.