Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina.
作者： Yuelan Zhao , Chengmin Wang , Yanmin Lu , Said Amer , Ping Xu
DOI: 10.1007/S00436-011-2381-9
关键词:
摘要: Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E amplified and cloned into pET28a(+) vector then transformed Escherichia coli BL21 strain. Results showed that fusion protein band about 22 kDa identified by SDS-PAGE. Western blot analysis indicated the recombinant specifically reacted with E. polyclonal antibody.
springer.com
sci-hub.se 参考文章(34)
Bao YongZhan, Zhao YueLan, Qin JianHua, Gao Ying, Lu JunXia, Zhu YuTao, Cloning and sequence analysis of 3-1E gene of merozoites of Eimeria acervulina Baoding strain isolated from Hebei province of China. Chinese journal of veterinary science. ,vol. 29, pp. 1278- 1281 ,(2009)
K S Kim, M C Jenkins, H S Lillehoj, Immunization of chickens with live Escherichia coli expressing Eimeria acervulina merozoite recombinant antigen induces partial protection against coccidiosis Infection and Immunity. ,vol. 57, pp. 2434- 2440 ,(1989) , 10.1128/IAI.57.8.2434-2440.1989
M S Crane, B Goggin, R M Pellegrino, O J Ravino, C Lange, Y D Karkhanis, K E Kirk, P R Chakraborty, Cross-protection against four species of chicken coccidia with a single recombinant antigen. Infection and Immunity. ,vol. 59, pp. 1271- 1277 ,(1991) , 10.1128/IAI.59.4.1271-1277.1991
Hyun S. Lillehoj, James M. Trout, Coccidia: a review of recent advances on immunity and vaccine development. Avian Pathology. ,vol. 22, pp. 3- 31 ,(1993) , 10.1080/03079459308418897
Mohammad Ali A. Shah, Roufeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li, A recombinant DNA vaccine encoding Eimeria acervulina cSZ-2 induces immunity against experimental E. tenella infection. Veterinary Parasitology. ,vol. 169, pp. 185- 189 ,(2010) , 10.1016/J.VETPAR.2009.12.035
Hyun S. Lillehoj, M. D. Ruff, Comparison of disease susceptibility and subclass-specific antibody response in SC and FP chickens experimentally inoculated with Eimeria tenella, E. acervulina, or E. maxima. Avian Diseases. ,vol. 31, pp. 112- 119 ,(1987) , 10.2307/1590782
C.C. Constantinoiu, J.B. Molloy, W.K. Jorgensen, G.T. Coleman, Characterization of the antibody response in birds following infection with wild-type and attenuated strains of Eimeria tenella and Eimeria necatrix. Veterinary Parasitology. ,vol. 175, pp. 47- 51 ,(2011) , 10.1016/J.VETPAR.2010.09.025
João Luis Garcia, José da Silva Guimarães, Selwyn Arlington Headley, Alexey Leon Gomel Bogado, Felipe Monteiro Bugni, Daniela Cristina Ramalho, Leonardo Montemor de Souza, Eimeria tenella: utilization of a nasal vaccine with sporozoite antigens incorporated into Iscom as protection for broiler breeders against a homologous challenge. Experimental Parasitology. ,vol. 120, pp. 185- 190 ,(2008) , 10.1016/J.EXPPARA.2008.07.007
Hongyan Song, Ruofeng Yan, Lixin Xu, Xiaokai Song, Muhammad Ali A Shah, Huili Zhu, Xiangrui Li, Efficacy of DNA vaccines carrying Eimeria acervulina lactate dehydrogenase antigen gene against coccidiosis Experimental Parasitology. ,vol. 126, pp. 224- 231 ,(2010) , 10.1016/J.EXPPARA.2010.05.015
Michael G. Wallach, Udi Ashash, Amnon Michael, Nicholas C. Smith, Field Application of a Subunit Vaccine against an Enteric Protozoan Disease PLoS ONE. ,vol. 3, pp. e3948- ,(2008) , 10.1371/JOURNAL.PONE.0003948