Structural-perturbation approaches to thermodynamics of site-specific protein-DNA interactions.

摘要: Publisher Summary This chapter presents the structural-perturbation approaches to thermodynamics of site-specific protein-DNA interactions. The conceptual foundation structural perturbation methods derives from idea a thermodynamic cycle, referred as pseudocycle because some constituent steps represent imaginary chemical transformations. In methods, values K obs are determined for normal DNA site and one or more modified DNAs proteins. As an introduction kinds information that may be derived energetic factors contribute interaction between protein its recognition is enumerated. set discussed, when used in concert with variety measures thermodynamic, kinetic, parameters, allows gaining significant insights into behavior protein- complexes. Determination either kinetic parameters DNA-protein interactions requires accurate knowledge molar concentration active protein.