Salmonella Typhimurium resides largely as an extracellular pathogen in porcine tonsils, independently of biofilm-associated genes csgA, csgD and adrA

摘要: Abstract Persistent Salmonella Typhimurium infections in pigs are a major concern for food safety and human health. Tonsils play key role the persistence of pigs. Previous studies indicated that virulence genes involved invasion intracellular survival little importance colonization porcine tonsils, suggesting predominantly extracellular location bacteria. Biofilm formation might promote Typhimurium. The aim this study was to determine whether bacterium resides intra- or extracellularly tonsils examine contribution biofilm-associated csgA , csgD adrA tonsils. Single cell suspensions were prepared from orally inoculated (2 × 10 7  colony forming units (CFU) wild type Typhimurium) ratio versus Both at 5 28 days post-inoculation (pi), majority bacteria found To biofilm persistence, with mixture 2 × 10  CFU strain one mutants. At 10 pi, equal numbers both mutant not only but also ileum, ileum contents, ileocecal lymph nodes faeces. In conclusion, we showed using independent mechanism.