Immunological localization of cystic fibrosis candidate gene products.
作者： A.T. Hoogeveen , J. Keulemans , R. Willemsen , B.J. Scholte , J. Bijman
DOI: 10.1016/0014-4827(91)90118-E
关键词:
摘要: Abstract The recent identification of the cystic fibrosis (CF) gene and its putative protein product, CF transmembrane conductance regulator (CFTR), enabled us to synthesize oligopeptides corresponding with a predicted extracellular domain (position 103–117; peptide A) cytoplasmic 501–515; B) constituting phenylalanine deletion (F 508) observed in majority mutations. Immunobiochemical studies antibodies directed against these peptides revealed presence two CFTR candidate proteins (155 195 kDa) various types epithelial cells. Immunolocalization performed on slices human duodenum showed strongest expression endoplasmic reticulum (RER) mucusproducing Goblet Labeling is also demonstrated RER apical membranes villus crypt cells, however, weaker extent.
elsevier.com
sci-hub.se 参考文章(13)
Bob J. Scholte, Maarten Kansen, Andre T. Hoogeveen, Rob Willemse, Johng S. Rhim, Arthur W.M. Van Der Kamp, Jan Bijman, Immortalization of nasal polyp epithelial cells from cystic fibrosis patients. Experimental Cell Research. ,vol. 182, pp. 559- 571 ,(1989) , 10.1016/0014-4827(89)90259-0
Johanna M Rommens, Michael C Iannuzzi, Bat-sheva Kerem, Mitchell L Drumm, Georg Melmer, Michael Dean, Richard Rozmahel, Jeffery L Cole, Dara Kennedy, Noriko Hidaka, Martha Zsiga, Manuel Buchwald, Lap-Chee Tsui, John R Riordan, Francis S Collins, Identification of the cystic fibrosis gene: chromosome walking and jumping Science. ,vol. 245, pp. 1059- 1065 ,(1989) , 10.1126/SCIENCE.2772657
C.G. O'Connor, L.K. Ashman, Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures Journal of Immunological Methods. ,vol. 54, pp. 267- 271 ,(1982) , 10.1016/0022-1759(82)90068-0
Mitchell L. Drumm, Heidi A. Pope, William H. Cliff, Johanna M. Rommens, Sheila A. Marvin, Lap-Chee Tsui, Francis S. Collins, Raymond A. Frizzell, James M. Wilson, Correction of the cystic fibrosis defect in vitro by retrovirus-mediated gene transfer. Cell. ,vol. 62, pp. 1227- 1233 ,(1990) , 10.1016/0092-8674(90)90398-X
Devra P. Rich, Matthew P. Anderson, Richard J. Gregory, Seng H. Cheng, Sucharita Paul, Douglas M. Jefferson, John D. McCann, Katherine W. Klinger, Alan E. Smith, Michael J. Welsh, Expression of cystic fibrosis transmembrane conductance regulator corrects defective chloride channel regulation in cystic fibrosis airway epithelial cells. Nature. ,vol. 347, pp. 358- 363 ,(1990) , 10.1038/347358A0
Carol Featherstone, An ATP-driven pump for secretion of yeast mating factor Trends in Biochemical Sciences. ,vol. 15, pp. 169- 170 ,(1990) , 10.1016/0968-0004(90)90151-Z
Richard J. Gregory, Seng H. Cheng, Devra P. Rich, John Marshall, Sucharita Paul, Kathleen Hehir, Lynda Ostedgaard, Katherine W. Klinger, Michael J. Welsh, Alan E. Smith, Expression and characterization of the cystic fibrosis transmembrane conductance regulator. Nature. ,vol. 347, pp. 382- 386 ,(1990) , 10.1038/347382A0
Riordan, J. Rommens, B Kerem, N Alon, R Rozmahel, Z Grzelczak, J Zielenski, S Lok, N Plavsic, J. Chou, al. et, Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science. ,vol. 245, pp. 1066- 1073 ,(1989) , 10.1126/SCIENCE.2475911
Stephen C. Hyde, Paul Emsley, Michael J. Hartshorn, Michael M. Mimmack, Uzi Gileadi, Stephen R. Pearce, Maurice P. Gallagher, Deborah R. Gill, Roderick E. Hubbard, Christopher F. Higgins, Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport. Nature. ,vol. 346, pp. 362- 365 ,(1990) , 10.1038/346362A0
R Willemsen, JM Van Dongen, JMFG Aerts, AW Schram, JM Tager, R Goudsmit, AJJ Reuser, None, An immunoelectron microscopic study of glucocerebrosidase in type 1 Gaucher's disease spleen. Ultrastructural Pathology. ,vol. 12, pp. 471- 478 ,(1988) , 10.3109/01913128809032232