mRNA expression and DNA methylation analysis of the inhibitory mechanism of H 2 O 2 on the proliferation of A549 cells

摘要: Reactive oxygen species, particularly hydrogen peroxide (H2O2), can induce proliferation inhibition and death of A549 cells via oxidative stress. Oxidative stress has effect on DNA methylation. methylation feature a common denominator: The one carbon cycle. To explore the inhibitory mechanism H2O2 lung cancer cells, present study analysed mRNA expression profiles in treated with for 24 h, as adenocarcinoma is most pathological type cancer. profile was constructed using reduced representation bisulphite sequencing, which identified 29,755 differentially methylated sites (15,365 upregulated 14,390 downregulated), 1,575 regions located gene promoters were methylKit. Analysis assocaition between levels revealed that several genes downregulated hypermethylated, including cyclin-dependent kinase inhibitor 3, denticleless E3 ubiquitin protein ligase homolog, centromere (CENP)F, kinesin family member (KIF)20A, CENPA, KIF11, PCNA clamp-associated factor GINS complex subunit 2, may be involved process cells.