The association of Western flower thrips, Frankliniella occidentalis, with a near Erwinia species gut bacteria: transient or permanent?

摘要: Associations between insects and gut bacteria are ubiquitous. It is possible to make a distinction permanent associations (called symbiosis), in which the same type of present more than one generation insect, transient associations. Transient ingested together with food but do not settle insect such way that they will be passed on next generation. In this study, we describe association Western flower thrips (Frankliniella occidentalis), polyphagous species major pest worldwide, bacteria. On basis direct microscopic observations counts bacteria, it was found from populations studied contained large numbers their hindgut. Bacteria were isolated host grown 10 different agar media. The number media equaled counts. All isolates had colony morphology. 16S rDNA sequence these identified as Enterobacteriaceae, closely related Escherichia coli. Isolates tested API 20E biochemical tests Erwinia species. This only all individuals any Universal primers, would potentially pick up DNA bacterium applied crude extracts We sequences similar those life stages persist for at least 2 years (more 50 generations).