Reply: Regulation of human adipose-derived stromal cell osteogenic differentiation by insulin-like growth factor-1 and platelet-derived growth factor-α

摘要: BACKGROUND Human adipose-derived stromal cells possess a great potential for tissue engineering purposes. The authors' laboratory is interested in harnessing human skeletal regeneration and identifying those factors that enhance cell osteogenic differentiation. authors hypothesized insulin-like growth factor (IGF) platelet-derived (PDGF) would stimulate osteogenesis IGF adipogenesis. METHODS Adipose-derived were harvested from lipoaspirate. Previously, microarray analysis examined gene expression throughout In candidate fashion, the added recombinant IGF-1 PDGF-alpha individually combination. Osteogenesis adipogenesis assessed by alkaline phosphatase, alizarin red, oil red O staining, quantitative real-time polymerase chain reaction (RUNX2, ALP, OCN, IGF1, PPARG, LPL, AP2, GCP1). Finally, intersection between PDGF signaling pathways was evaluated. RESULTS observed to increase differentiation all markers (p < 0.01). However, when alone primarily did not affect markers. positively regulated transcription of IGF1. Addition combination with or before enhanced more than either alone. increased whereas diminished CONCLUSIONS significantly may be used tissue-engineering beneficial driving osteogenesis. Future vivo applications will focus on cells, biomimetic scaffolds, IGF.