Cellular processing of murine colony-stimulating factor (Multi-CSF, GM-CSF, G-CSF) receptors by normal hemopoietic cells and cell lines.

摘要: The binding, internalization and degradation rates of three different murine colony-stimulating factors (Multi-CSF or interleukin-3, GM-CSF G-CSF) their receptor turnover were determined for normal bone marrow cells a number cell lines at 37 degrees C. kinetic parameters extracted from curve-fitting analysis the approach to steady-state surface-bound internalized CSFs by methods described Myers et al. (1987). primary binding constants (association dissociation) each CSF on types similar, suggesting single type CSF. In all cases, induced faster rate occupied receptors than unoccupied resulted in significant accumulation inside under conditions. constant, determining relationship between concentration occupancy, indicated that, more given conditions would be equilibrium Nevertheless, data predicted that maximal biological effects exerted concentrations did not result full occupancy. Comparison derived same interacting with processing dynamic interplay receptor-determined cell-determined events. This flexibility matched variety responses elicited types.