Establishment of NOD/SCID mouse models of human hepatocellular carcinoma via subcutaneous transplantation of histologically intact tumor tissue
作者： Mingxia Yan , Ming Yao , Chao Ge , Fangyu Zhao , Jinjun Li
DOI: 10.3978/J.ISSN.1000-9604.2013.05.02
关键词:
摘要: Hepatocellular carcinoma (HCC) is one of the most deadly human cancers, but it very difficult to establish an animal model by using surgical specimens. In present experiment, histologically intact fresh specimens HCC were subcutaneously transplanted in non-obese diabetic/severe combined immunodeficienccy (NOD/SCID) mice. The biological characteristics original and corresponding tumors cell lines investigated. results showed that 5 new models 2 primary successfully established from Hematoxylin-eosin staining xenografts retained major histological features two had been cultivated for 3 years successively passaged more than 100 passages vitro. morphological biologic genetically similar tumor. subcutaneous transplant with tumor tissue could be useful vivo vitro testing anti-cancer drugs ideal study various HCC.
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