p53 Amino Acids 339–346 Represent the Minimal p53 Repression Domain
作者： Tse-Ming Hong , Jeremy J. W. Chen , Konan Peck , Pan-Chyr Yang , Cheng-Wen Wu
关键词:
摘要: The p53 tumor suppressor protein functions as an activator and also a repressor of gene transcription. Currently, the mechanism transcriptional repression by remains poorly understood. To help clarify this mechanism, we carried out studies designed to identify minimal domain that inhibits activities. We found only eight amino acids (339) COOH-terminal (termed P53MRD) possess activities repression. exact location is on E6-binding region, it lacks ability tetramerization. P53MRD able repress transcription while not affecting VP16. mutants (amino M340P F341D) native lost thymidine kinase chloramphenicol acetyltransferase promoter. These results suggest eight-amino acid element required for p53.
sci-hub.se 参考文章(44)
Brown Tf, Malkin D, Sinclair Ae, Douglass Ec, Jolly Kw, Look At, Splice-site mutation of the p53 gene in a family with hereditary breast-ovarian cancer Oncogene. ,vol. 9, pp. 97- 102 ,(1994)
C. Moyret-Lalle, S. Mazoyer, H. Sobol, M. Ozturk, P. Lalle, Didier Frappaz, S. Schraub, C. Marcais, Two germ-line mutations affecting thé same nucleotide at codon 257 of p53 gène, a rare site for mutations Oncogene. ,vol. 9, pp. 1237- 1239 ,(1994)
Pellegata Ns, Stanbridge Ej, Cajot Jf, The basic carboxy-terminal domain of human p53 is dispensable for both transcriptional regulation and inhibition of tumor cell growth. Oncogene. ,vol. 11, pp. 337- 349 ,(1995)
M A Subler, D W Martin, S Deb, Inhibition of viral and cellular promoters by human wild-type p53. Journal of Virology. ,vol. 66, pp. 4757- 4762 ,(1992) , 10.1128/JVI.66.8.4757-4762.1992
T. Unger, M.M. Nau, S. Segal, J.D. Minna, p53: a transdominant regulator of transcription whose function is ablated by mutations occurring in human cancer. The EMBO Journal. ,vol. 11, pp. 1383- 1390 ,(1992) , 10.1002/J.1460-2075.1992.TB05183.X
Yu-Shen Hsu, Fen-Mei Tang, Wei-Li Liu, Jing-Yuan Chuang, Ming-Yang Lai, Young-Sun Lin, Transcriptional Regulation by p53 Journal of Biological Chemistry. ,vol. 270, pp. 6966- 6974 ,(1995) , 10.1074/JBC.270.12.6966
Mauro F. Santibáñez-Koref, Karen J. Tricker, Alison Condie, Derek Crowther, Jillian M. Birch, D. Gareth R. Evans, Ann L. Hartley, Jane Prosser, Alan W. Craft, Eileen Thompson, John Martin, Martin Harris, Patricia H. Morris Jones, Osborne B. Eden, Aine Binchy, Anna M. Kelsey, Jillian R. Mann, Erika L. D. Mitchell, Prevalence and diversity of constitutional mutations in the p53 gene among 21 Li-Fraumeni families. Cancer Research. ,vol. 54, pp. 1298- 1304 ,(1994)
W D Funk, D T Pak, R H Karas, W E Wright, J W Shay, A transcriptionally active DNA-binding site for human p53 protein complexes. Molecular and Cellular Biology. ,vol. 12, pp. 2866- 2871 ,(1992) , 10.1128/MCB.12.6.2866
Yang Shi, Edward Seto, Long-Sheng Chang, Thomas Shenk, Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell. ,vol. 67, pp. 377- 388 ,(1991) , 10.1016/0092-8674(91)90189-6
Jing-Yuan Chuang, Chin-Tarng Lin, Cheng-Wen Wu, Young-Sun Lin, A movable and regulable inactivation function within the central region of a temperature-sensitive p53 mutant Journal of Biological Chemistry. ,vol. 270, pp. 23899- 23902 ,(1995) , 10.1074/JBC.270.41.23899