In Search of the Optimal Experimental Model

摘要: The increasing concern that environmental exposures may affect mammary gland differentiation and their potential for cancer risk has placed greater emphasis on the standardization of morphometric methods evaluation development in experimental animal models. Traditional morphological studies whole mount preparations histological sections have provided an enormous amount information degree its response to diet, endogenous exogenous hormones, chemicals, xenoestrogens, or physical agents. Their usefulness assessing effect at specific stages growth will depend upon optimal specimen selection data comparisons among laboratories. Past present work clearly indicated study rat under stereomicroscope, combined with microscopic analysis, represents important tool evaluating assessment. identification terminal end bud glands rodents intermediate cell (IC) as targets carcinogens indicate adequate quantification these structures during windows would provide sufficient carcinogenic response. This classical model serves a useful incorporating state-of-the-art technologies permit microdissection stem cells from location sites genomic proteomic analysis. Knowledge gained through serve predicting probabilities developing by detecting early epigenetic damage occurring progenitor cells.