Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test).

摘要: Abstract SYBR® Green I nucleic acid gel stain is an unsymmetrical cyanine dye developed for sensitive detection of acids in electrophoretic gels. Its mechanism binding not known, whereas the most commonly used stain, ethidium bromide, a well-characterized intercalator. We compared mutagenicity SYBR with that bromide Salmonella/mammalian microsome reverse mutation assays (Ames tests). As expected [J. McCann, E. Choi, Yamasaki, B.N. Ames, Proc. Natl. Acad. Sci. USA, 72 (1975) 5135–5139], showed high revertant frequencies several frameshift indicator strains (averaging 68-fold higher than vehicle controls TA98, 80-fold TA1538, 15-fold TA1537, and 4.4-fold TA97a), only presence rat liver extracts (S9). Small increases were observed base-substitution strain TA102 both absence S9 2.0- 1.8-fold controls, respectively) TA100 1.6-fold controls). A small mutagenic effect was detected TA98 2.2-fold controls) TA102, 2.2- 2.7-fold respectively). Thus, weak mutagen appears to be much less bromide. These results suggest may intercalate, if it does, its does give rise point mutations at frequency.