A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae.

摘要: Respiration-defective pet mutants of Saccharomyces cerevisiae, assigned to complementation group G25, are grossly deficient in mitochondrial respiratory and ATPase complexes. This phenotype is usually found strains impaired protein synthesis. The G25 mutants, however, synthesize all the proteins encoded by DNA. also able import process cytoplasmically derived subunits these enzymes. These results most compatible with idea that gene defined (RCA1) codes for a essential assembly functional RCA1 has been cloned an rca1 mutant yeast genomic library. sequence product shows Rca1 be new member recently described family ATPases. membrane third known this implicated function biogenesis mitochondria. primary structure indicates several distinct domains addition common purine nucleotide binding region shared members family. One, located amino-terminal half, contains two hydrophobic stretches sufficient length span lipid bilayer.