Electrochemiluminescence imaging for parallel single-cell analysis of active membrane cholesterol.

作者: Junyu Zhou , Guangzhong Ma , Yun Chen , Danjun Fang , Dechen Jiang

DOI: 10.1021/ACS.ANALCHEM.5B00542

关键词:

摘要: Luminol electrochemiluminescence (ECL) imaging was developed for the parallel measurement of active membrane cholesterol at single living cells, thus establishing a novel electrochemical detection technique for single cells with high analysis throughput and low detection limit. In our strategy, the luminescence generated from luminol and hydrogen peroxide upon the potential was recorded in one image so that hydrogen peroxide at the surface of multiple cells could be simultaneously analyzed. Compared with the classic …

参考文章(34)
Y. Lange, H.B. Cutler, T.L. Steck, The effect of cholesterol and other intercalated amphipaths on the contour and stability of the isolated red cell membrane. Journal of Biological Chemistry. ,vol. 255, pp. 9331- 9337 ,(1980) , 10.1016/S0021-9258(19)70566-3
Y. Lange, J. Ye, T. L. Steck, How cholesterol homeostasis is regulated by plasma membrane cholesterol in excess of phospholipids Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 11664- 11667 ,(2004) , 10.1073/PNAS.0404766101
Milica Sentic, Milena Milutinovic, Frédéric Kanoufi, Dragan Manojlovic, Stéphane Arbault, Neso Sojic, Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays Chemical Science. ,vol. 5, pp. 2568- 2572 ,(2014) , 10.1039/C4SC00312H
Xin Liu, Syed Barizuddin, Wonchul Shin, Cherian J. Mathai, Shubhra Gangopadhyay, Kevin D. Gillis, Microwell device for targeting single cells to electrochemical microelectrodes for high-throughput amperometric detection of quantal exocytosis. Analytical Chemistry. ,vol. 83, pp. 2445- 2451 ,(2011) , 10.1021/AC1033616
Linru Xu, Zhenyu Zhou, Congzhe Zhang, Yayun He, Bin Su, Electrochemiluminescence imaging of latent fingermarks through the immunodetection of secretions in human perspiration Chemical Communications. ,vol. 50, pp. 9097- 9100 ,(2014) , 10.1039/C4CC03466J
Frédérique Deiss, Christopher N. LaFratta, Matthew Symer, Timothy M. Blicharz, Neso Sojic, David R. Walt, Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level. Journal of the American Chemical Society. ,vol. 131, pp. 6088- 6089 ,(2009) , 10.1021/JA901876Z
Wujian Miao, Electrogenerated Chemiluminescence and Its Biorelated Applications Chemical Reviews. ,vol. 108, pp. 2506- 2553 ,(2008) , 10.1021/CR068083A
Jun Wang, Raphaël Trouillon, Johan Dunevall, Andrew G. Ewing, Spatial Resolution of Single-Cell Exocytosis by Microwell-Based Individually Addressable Thin Film Ultramicroelectrode Arrays Analytical Chemistry. ,vol. 86, pp. 4515- 4520 ,(2014) , 10.1021/AC500443Q
Yoriko Iwanaga, Dieter Braun, Peter Fromherz, No correlation of focal contacts and close adhesion by comparing GFP-vinculin and fluorescence interference of DiI European Biophysics Journal. ,vol. 30, pp. 17- 26 ,(2001) , 10.1007/S002490000119