Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug.
作者： Gregory Marslin , Vinoth Khandelwal , Gregory Franklin
DOI: 10.2147/NSA.S254770
关键词:
摘要: Purpose Cordycepin, a natural product isolated from the fungus Cordyceps militaris, is potential candidate for breast cancer therapy. However, due to its structural similarity with adenosine, cordycepin rapidly metabolized into an inactive form in body, hindering development as therapeutic agent. In present study, we have prepared nanoparticles poly(lactic-co-glycolic acid) (PLGA) and compared their cellular uptake, cytotoxicity hemolytic free cordycepin. Materials methods Cordycepin-loaded PLGA (CPNPs) were by double-emulsion solvent evaporation method. Physico-chemical characterization of was done zetasizer, transmission electron microscopy (TEM) reverse-phase high-pressure liquid chromatography (RP-HPLC) analyses. Cellular uptake CPNPs drug tested human cells (MCF7). Hemolytic both these forms evaluated rat red blood (RBCs). Results revealed that spherical shape, possessed size range 179-246 nm, released encapsulated sustainably over period 10 days. exhibited high level than MCF-7 cells. While not toxic RBCs even at concentrations, induced hemolysis relatively low concentration. Conclusion Our results reveal delivery could enhance clinical efficacy substantially.
sci-hub.st 参考文章(36)
Hyun-Jeong Cho, Jae-Youl Cho, Man-Hee Rhee, Hyeong-Soo Kim, Hyun-Sub Lee, Hwa-Jin Park, None, Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. Journal of Microbiology and Biotechnology. ,vol. 17, pp. 1134- 1138 ,(2007)
Gregory Marslin, Ann Mary Revina, Vinoth Kumar Megraj Khandelwal, Krishnamoorthy Balakumar, Caroline J Sheeba, Gregory Franklin, None, PEGylated ofloxacin nanoparticles render strong antibacterial activity against many clinically important human pathogens Colloids and Surfaces B: Biointerfaces. ,vol. 132, pp. 62- 70 ,(2015) , 10.1016/J.COLSURFB.2015.04.050
Michael R. Blackburn, Rodney E. Kellems, Adenosine deaminase deficiency: metabolic basis of immune deficiency and pulmonary inflammation. Advances in Immunology. ,vol. 86, pp. 1- 41 ,(2005) , 10.1016/S0065-2776(04)86001-2
XUEWEN TIAN, YUJIAN LI, YINYU SHEN, QIAOQIAO LI, QINGLU WANG, LIANSHI FENG, Apoptosis and inhibition of proliferation of cancer cells induced by cordycepin. Oncology Letters. ,vol. 10, pp. 595- 599 ,(2015) , 10.3892/OL.2015.3273
Sang Seung Lee, Yong Bok Lee, In Joon Oh, Cellular uptake of poly(dl-lactide-co-glycolide) nanoparticles: effects of drugs and surface characteristics of nanoparticles Journal of Pharmaceutical Investigation. ,vol. 45, pp. 659- 667 ,(2015) , 10.1007/S40005-015-0221-0
Xie Huichun, Yong Liang, Deqiang Lao, Tianyou Zhang, Yoichiro Ito, Preparative separation of high-purity cordycepin from Cordyceps militaris(L.) Link by high-speed countercurrent chromatography. Journal of Liquid Chromatography & Related Technologies. ,vol. 37, pp. 491- 499 ,(2011) , 10.1080/10826076.2011.556965
Kiran R Chaudhari, Abhinesh Kumar, Vinoth Kumar Megraj Khandelwal, Anil K Mishra, Jukka Monkkonen, Rayasa S Ramachandra Murthy, None, Targeting Efficiency and Biodistribution of Zoledronate Conjugated Docetaxel Loaded Pegylated PBCA Nanoparticles for Bone Metastasis Advanced Functional Materials. ,vol. 22, pp. 4101- 4114 ,(2012) , 10.1002/ADFM.201102357
Gen Li, Izumi Nakagome, Shuichi Hirono, Tomoo Itoh, Ryoichi Fujiwara, Inhibition of adenosine deaminase (ADA)‐mediated metabolism of cordycepin by natural substances Pharmacology Research & Perspectives. ,vol. 3, ,(2015) , 10.1002/PRP2.121
Kiran Ramanlal Chaudhari, Abhinesh Kumar, Vinoth Kumar Megraj Khandelwal, Mukesh Ukawala, Arehalli S. Manjappa, Anil Kumar Mishra, Jukka Monkkonen, Rayasa S. Ramachandra Murthy, Bone metastasis targeting: a novel approach to reach bone using Zoledronate anchored PLGA nanoparticle as carrier system loaded with Docetaxel. Journal of Controlled Release. ,vol. 158, pp. 470- 478 ,(2012) , 10.1016/J.JCONREL.2011.11.020
Sijing XIONG, Xinxin Zhao, Boon Chin Heng, Kee Woei Ng, Joachim Say-Chye Loo, Cellular uptake of Poly-(D,L-lactide-co-glycolide) (PLGA) nanoparticles synthesized through solvent emulsion evaporation and nanoprecipitation method. Biotechnology Journal. ,vol. 6, pp. 501- 508 ,(2011) , 10.1002/BIOT.201000351