Amperometric choline biosensor based on multiwalled carbon nanotubes/zirconium oxide nanoparticles electrodeposited on glassy carbon electrode.
作者： S. Pundir , N. Chauhan , J. Narang , C.S. Pundir
关键词:
摘要: A bienzymatic choline biosensor was constructed by coimmobilizing acetylcholinesterase (AChE) and oxidase (ChO) onto nanocomposite of carboxylated multiwalled carbon nanotubes (c-MWCNTs) zirconium oxide nanoparticles (ZrO(2)NPs) electrodeposited on the surface a glassy electrode (GCE) using it (AChE-ChO/c-MWCNT/ZrO(2)NPs/GCE) as working electrode, Ag/AgCl reference Pt wire auxiliary connected through potentiostat. The enzyme characterized scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV) studies, optimized, evaluated. exhibited optimum response within 4 s at +0.2V, pH 7.4, 25 °C. detection limit range were 0.01 μM 0.05 to 200 μM, respectively. half-life 60 days serum level, measured biosensor, 9.0 12.8 μmol/L (with mean 10.81 μmol/L) in apparently healthy persons 5.0 8.4 6.53 suffering from Alzheimer's disease. unaffected number substances.
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