Analysis of blood-induced Anopheles gambiae midgut proteins and sexual stage Plasmodium falciparum interaction reveals mosquito genes important for malaria transmission.
作者： Yingjun Cui , Guodong Niu , Vincent L. Li , Xiaohong Wang , Jun Li
DOI: 10.1038/S41598-020-71186-5
关键词:
摘要: Plasmodium invasion of mosquito midguts is a mandatory step for malaria transmission. The roles midgut proteins and parasite interaction during transmission are not clear. This study aims to identify that interact with affect P. falciparum invasion. Based on gene expression profiles protein sequences, 76 secretory highly expressed in up-regulated by blood meals were chosen analysis. About 61 candidate genes successfully cloned from Anopheles gambiae insect cells. ELISA analysis showed 25 the cell-expressed recombinant interacted falciparum-infected cell lysates. Indirect immunofluorescence assays confirmed 17 them sexual stage parasites significantly stronger than asexual parasites. Knockdown found seven changed mosquitoes' susceptibility falciparum. Four (AGAP006268, AGAP002848, AGAP006972, AGAP002851) played protective function against invasion, other three (AGAP008138, FREP1, HPX15) facilitated mosquitoes. Notably, AGAP008138 unique only exists Anopheline These products ideal targets block
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