A novel mechanism of sugar selection utilized by a human X-family DNA polymerase.
作者： Jessica A. Brown , Kevin A. Fiala , Jason D. Fowler , Shanen M. Sherrer , Sean A. Newmister
DOI: 10.1016/J.JMB.2009.11.003
关键词:
摘要: During DNA synthesis, most polymerases and reverse transcriptases select against ribonucleotides via a steric clash between the ribose 2'-hydroxyl group bulky side chain of an active-site residue. In this study, we demonstrated that human polymerase lambda used novel sugar selection mechanism to discriminate ribonucleotides, whereby was excluded mostly by backbone segment slightly Y505. Such further be dependent on size orientation substituent covalently attached at ribonucleotide C2'-position.
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sci-hub.se 参考文章(27)
Ana Bonnin, José M Lázaro, Luis Blanco, Margarita Salas, A single tyrosine prevents insertion of ribonucleotides in the eukaryotic-type φ29 DNA polymerase Journal of Molecular Biology. ,vol. 290, pp. 241- 251 ,(1999) , 10.1006/JMBI.1999.2900
Kristijan Ramadan, Giovanni Maga, Igor V Shevelev, Giuseppe Villani, Luis Blanco, Ulrich Hübscher, Human DNA Polymerase λ Possesses Terminal Deoxyribonucleotidyl Transferase Activity And Can Elongate RNA Primers: Implications for Novel Functions Journal of Molecular Biology. ,vol. 328, pp. 63- 72 ,(2003) , 10.1016/S0022-2836(03)00265-1
Miguel Garcı́a-Dı́az, Katarzyna Bebenek, Thomas A. Kunkel, Luis Blanco, Identification of an Intrinsic 5′-Deoxyribose-5-phosphate Lyase Activity in Human DNA Polymerase λ A POSSIBLE ROLE IN BASE EXCISION REPAIR Journal of Biological Chemistry. ,vol. 276, pp. 34659- 34663 ,(2001) , 10.1074/JBC.M106336200
Kalyan Das, Stefan G. Sarafianos, Arthur D. Clark, Paul L. Boyer, Stephen H. Hughes, Eddy Arnold, Crystal Structures of Clinically Relevant Lys103Asn/Tyr181Cys Double Mutant HIV-1 Reverse Transcriptase in Complexes with ATP and Non-nucleoside Inhibitor HBY 097. Journal of Molecular Biology. ,vol. 365, pp. 77- 89 ,(2007) , 10.1016/J.JMB.2006.08.097
H Pelletier, M. Sawaya, A Kumar, S. Wilson, J Kraut, Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP Science. ,vol. 264, pp. 1891- 1903 ,(1994) , 10.1126/SCIENCE.7516580
C. M. Joyce, Choosing the right sugar: how polymerases select a nucleotide substrate. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 1619- 1622 ,(1997) , 10.1073/PNAS.94.5.1619
Jean-Baptiste Boulé, François Rougeon, Catherine Papanicolaou, Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. Journal of Biological Chemistry. ,vol. 276, pp. 31388- 31393 ,(2001) , 10.1074/JBC.M105272200
Jean-Pascal Capp, François Boudsocq, Pascale Bertrand, Audrey Laroche-Clary, Philippe Pourquier, Bernard S Lopez, Christophe Cazaux, Jean-Sebastien Hoffmann, Yvan Canitrot, The DNA polymerase λ is required for the repair of non-compatible DNA double strand breaks by NHEJ in mammalian cells Nucleic Acids Research. ,vol. 34, pp. 2998- 3007 ,(2006) , 10.1093/NAR/GKL380
Thomas W. Traut, Physiological concentrations of purines and pyrimidines. Molecular and Cellular Biochemistry. ,vol. 140, pp. 1- 22 ,(1994) , 10.1007/BF00928361
Andrew F Gardner, William E Jack, Determinants of nucleotide sugar recognition in an archaeon DNA polymerase Nucleic Acids Research. ,vol. 27, pp. 2545- 2553 ,(1999) , 10.1093/NAR/27.12.2545