The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog.

摘要: A human myeloid transcript of approximately 4 kilobases was cloned as a cDNA from an expression library based on homology with the guinea pig recently described by Honda et al. (Honda, Z-I., Nakamura, M., Miki, I., Minami, Watanabe, T., Seyama, Y., Okado, H., Tok, Ito, K., Miyamato, and Shimizu, T. (1991) Nature 349, 342-346) receptor for platelet-activating factor (PAF). The DNA confers high affinity binding sites when transfected into COS-7 cells has desensitization properties similar to leukocyte receptor. Southern analysis using this indicates that PAF gene is present single copy in genome. deduced protein sequence predicts seven hydrophobic regions receptor, characteristic rhodopsin family, 83% identical corresponding molecule. modified constructed inserting additional 30 nucleotides after 5'-ATG, encoding amino acid MDYKDDDDKEF, which specifically recognized monoclonal antibody. encodes functional detected antibody membrane cells. use construct supports structural model rhodopsin-like superfamily receptors places NH2-terminal extracellular side membrane, should additionally be useful purification protein.