Waxy strains of three amaranth grains raised by different mutations in the coding region

摘要: Waxy strains raised by waxy mutation have been found for three amaranth grains. Three genes encoding protein were isolated from Amaranthus caudatus (Wx-ca), A. cruentus (Wx-cr), and hypochondriacus (Wx-hy). Sequence analysis indicated that the Wx-ca, Wx-cr, Wx-hy contained same exon (13 exons) intron (12 introns) structure. The lengths of 3,236, 3,237, 3,225 bp, respectively. alignment coding sequence showed 12 polymorphic sites, including 11 single nucleotide polymorphisms (SNPs) (in exons 10 introns 1, 3, 4, 9, 11) 5 deletions or insertions (indels) 11). In particular, major polymorphism was detected in 8- 3-bp indels 4. Moreover, alleles (wx-ca, wx-cr, wx-hy) all species also characterized. Comparison sequences their one base insertion (wx-ca: insert T 8) a substitution (wx-cr: G-to-T 10; wx-hy: G-to-A 6), which occurred as internal termination codon genes, suggesting involvement nonsense frameshift mutation. Therefore, these different mutations regions considered to be cause (amylose-free) phenotype.