Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone.

摘要: A T cell clone (ACH-2) derived from cells infected with HIV-1 was found to produce in response stimulation a monokine-enriched supernatant prepared by culturing human monocyte/macrophages bacterial LPS (LPS-MO SN). Monokine induction of ACH-2 resulted augmented virus production reflected an increase reverse transcriptase activity and the synthesis all major viral proteins. Examination indirect immunofluorescence revealed that 10 15% uninduced constitutively expressed HIV proteins, whereas 100% showed positive LPS-MO SN. This SN approximately 100-fold infectious over cells. alone could not induce expression, greatest expression. Cell separation studies confirmed source inducing factor(s) be bearing mature monocyte/macrophage marker, Leu M3. Biochemical fractionation suggested one or more factors, having apparent Mr 45 kDa, were involved this induction. Absorption immunoaffinity gels specific for TNF-alpha shown completely remove line.