A simple and rapid solvent extraction method for determining total lipids in fish tissue

摘要: Solvent systems that have been developed for lipid extraction include chloroform-methanol, n-hexane-isopropyl alcohol, and methylene chloride-methanol. The methods are labor intensive, lack precision, or require a large volume of solvent. Correct computation content calls full recovery solvent after extraction, but always is incomplete because unaccounted residue remains in jar, filter paper, homogenized tissue. A rapid simple method coupled with correct was determining total lipids fish uses chloroform-methanol an Eberbach blending jar. Variables examined were ratio, solvent-to-sample phase separation time. Precision within 0.5%. Conventional depends on the chloroform measured filtration. This does not residue. Thus, time-consuming second required complete recovery. mass balance each filtration step confirmed (measured plus unaccounted) close to theoretical volume. procedure eliminates problems associated laborious variation readings exact reading Instead it allows use volume, which ratio used.