Chemical Probes to Directly Profile Palmitoleoylation of Proteins.
作者： Baohui Zheng , Gopala K. Jarugumilli , Baoen Chen , Xu Wu
关键词:
摘要: Palmitoleoylation is a unique fatty acylation of proteins in which monounsaturated acid, palmitoleic acid (C16:1), covalently attached to protein. Wnt are known be palmitoleoylated by cis-Δ9 palmitoleate at conserved serine residues. O-palmitoleoylation plays critical role regulating secretion, binding the receptors, and dynamics signaling. Therefore, protein palmitoleoylation important tissue homeostasis tumorigenesis. Chemical probes based on saturated acids, such as ω-alkynyl palmitic (Alk-14 or Alk-C16 ), have been used study palmitoleoylation. However, require prior conversion unsaturated stearoyl-CoA desaturase (SCD) cells, significantly decreasing their selectivity efficiency for studying We synthesized characterized cis- trans-palmitoleic acids (cis- trans-Alk-14:1) chemical directly found that cis-Alk-14:1 could more efficiently label cells. Interestingly, DHHC family palmitoyl acyltransferases can charge both potentially using acyl donors palmitoylation Furthermore, proteomic analysis targets labeled these revealed new trans-palmitoleoylated proteins. Our studies provided tools insights into cell
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