Adiposity and Age are Statistically Related to Enhanced RASSF1A Tumor Suppressor Gene Promoter Methylation in Normal Autopsy Kidney Tissue

摘要: Age, adiposity, and smoking are risk factors for the development of renal cell carcinoma. Hypermethylation RAS association domain family 1A gene (RASSF1A) promoter belongs to most frequently detected epigenetic alterations in human cancers including RASSF1A is functionally involved cycle control normal cells depletion promotes a number cellular changes increasing neoplastic growth. We investigated hypothesis that age, modulated by adiposity anthracosis as surrogate smoking, predictor methylation kidney tissue. Using cross-sectional study design, we quantitatively analyzed 78 autopsy tissues quantitative combined bisulfite restriction analysis sequencing, statistically evaluated degree relative relationship with age state anthracosis. Statistical showed (regression analysis; P < 0.001), (univariate = 0.016), (t test; 0.005) each significantly associated an increase However, only (P 0.008) were identified independent predictors using covariance analysis. This provides statistical evidence common cancer enhance nonmalignant