DICER: STRUCTURE, FUNCTION AND ROLE IN RNA-DEPENDENT GENE-SILENCING PATHWAYS

摘要: Dicer enzymes are double strand (ds)-specific RNases whose cleavage products used to provide specificity in RNA-based gene-silencing pathways. They encoded by the genomes of all multicellular and most unicellular eukaryotes. In mammals, is essential for viability. Evidence suggests that these modular 200 kD function cytoplasm conjunction with Dicer-binding proteins generate load small RNAs onto ribonucleoprotein complexes effect post-transcriptional gene-silencing. activity also required some types transcriptional nucleus. The importance as a central regulator gene expression illustrated recent estimates many one third human genes regulated (Lewis et al., 2005). this chapter, we discuss structure related