Determination of nitrofuran residues in milk of dairy cows using liquid chromatography-tandem mass spectrometry.

摘要: An analytical method has been developed for the determination of total bound and extractable residues nitrofuran drugs furazolidone, nitrofurazone, furaltadone, nitrofurantoin in milk dairy cows. The involves overnight acid hydrolysis simultaneous derivatization released side chains with 2-nitrobenzaldehyde. During hydrolysis, metabolites are hydrolyzed to chains. After pH adjustment solid-phase extraction cleanup, derivatives detected quantitated using a liquid chromatography−tandem mass spectrometry system an atmospheric pressure chemical ionization interface. Validation is accomplished by fortifying control mixture at 1, 2, 4 ng/g. Internal standards added beginning procedure compensate matrix effects recovery losses. Method accuracies range from 83 104% coefficients variation less than 13% all four analytes. limits detection ≤0.2 ng...