Characterization of rat calcitonin mRNA

摘要: Abstract A chimeric plasmic containing cDNA complementary to rat calcitonin mRNA has been constructed. Partial sequence analysis shows that the insert contains a nucleotide encoding complete amino acid of calcitonin. Two basic acids precede and three follow hormone sequence, suggesting is generated by proteolytic cleavage larger precursor in manner analogous other small polypeptide hormones. The COOH-terminal proline, known be amidated secreted hormone, followed glycine precursor. cloned DNA was used characterize expression mRNA. Cytoplasmic mRNAs from calcitonin-producing medullary thyroid carcinoma lines normal glands contain single species, 1050 nucleotides long, whch hybridizes cDNA. concentration sequences greater those tumors produce amounts immunoreactive RNAs endocrine tissues, including anterior neurointermediate lobes pituitary, no detectable