Yersinia pseudotuberculosis infection in breeding monkeys: detection and analysis of strain diversity by PCR.

摘要: In the last three decades, several monkeys reared in outdoor/indoor-outdoor breeding colonies and cages of Primate Research Institute, Kyoto University, died yersiniosis caused by Yersinia pseudotuberculosis, necessitating introduction a method to detect bacteria rapidly thus allow preventive measures be undertaken. A rapid nested polymerase chain reaction (PCR) for identification Y. pseudotuberculosis fecal samples random amplified polymorphic DNA (RAPD)-PCR approach distinguishing between bacterial strains were therefore developed. isolates from monkey specimens found classifiable into types. To determine source infection, hundreds wild rats, pigeons, sparrows collected around cages, subjected PCR analyses. was detected 1.7% rats. The fingerprints revealed RAPD-PCR same as that one strain isolated macaques, suggesting rat possible infection.