作者: Asmaa A. Sadoon , Prabhat Khadka , Jack Freeland , Ravi Kumar Gundampati , Ryan H. Manso
DOI: 10.1128/AEM.02479-19
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摘要: The antimicrobial activity and mechanism of silver ions (Ag+) have gained broad attention in recent years. However, dynamic studies are rare this field. Here, we report our measurement the effects Ag+ on dynamics histone-like nucleoid-structuring (H-NS) proteins live bacteria using single-particle-tracking photoactivated localization microscopy (sptPALM). It was found that treating with led to faster diffusive H-NS proteins. Several techniques were used understand observed dynamics. Electrophoretic mobility shift assay purified indicated weaken binding between DNA. Isothermal titration calorimetry confirmed DNA interact directly. Our recently developed sensing method based bent suggested caused dehybridization double-stranded (i.e., dissociation into single strands). These evidences us a plausible for when subjected ions: Ag+-induced weakens This work highlighted importance study cells understanding functions agents bacteria.IMPORTANCE As so-called "superbug" resistant commonly prescribed antibiotics become global threat public health years, noble metals, such as silver, various forms been attracting due their activities. most existing literature relied traditional bioassays studying silver; addition, temporal resolution is largely missing molecular inside bacteria. effect at nanoscale microscopy. highlights