Magnetic Resonance Imaging (MRI) of Intratumoral Voxel Heterogeneity as a Potential Response Biomarker: Assessment in a HER2+ Esophageal Adenocarcinoma Xenograft Following Trastuzumab and/or Cisplatin Therapy.

摘要: Abstract We evaluated magnetic resonance imaging (MRI) voxel heterogeneity following trastuzumab and/or cisplatin in a HER2+ esophageal xenograft (OE19) as potential response biomarker. OE19 xenografts treated with saline (controls), monotherapy, or combined and underwent 9.4-T MRI. Tumor MRI parametric maps of T1 relaxation time (pre/post contrast), T2 time, T2* rate (R2*), apparent diffusion coefficient obtained before (TIME0), after 24hours (TIME1), 2weeks treatment (TIME2) were analyzed. Voxel histogram fractal parameters (from the whole tumor, rim center, ratio rim‐to‐center) derived. Tumors stained for immunohistochemical markers hypoxia (CA-IX), angiogenesis (CD34), proliferation (Ki-67). Combination therapy reduced growth (relative change, ∆ +0.58±0.43 versus controls, +4.1±1.0; P =0.008). More spatially homogeneous distribution between to center was noted combination respectively, contrast-enhanced (90th percentile: 1.00 0.88, =0.009), (mean: 0.92, =0.006; median: 0.98 0.91, 75th 1.02 0.94, =0.007), R2* (10th 0.99 1.26, =0.003). found that monotherapy spatial compared control groups, former providing adjunctive tumor information.