Intrahepatic islet transplant in the mouse: functional and morphological characterization.

摘要: Although in a clinical setting islet transplantation is normally performed by percutaneous intrahepatic infusion, the kidney capsule has been site of choice nearly all studies using mice. In present study, we extensively characterized mouse model intraportally transplanted islets with purpose to propose it as study transplantation. C57BL/6 (n = 78) and BALB/C 53) recipients were 400 autologous alternatively through portal vein (PV-Tx) or under (KC-Tx). Glucose concentration during first hour after syngeneic infusion was associated subsequent long-term function confirming that early events have effects on graft function. both strains tested probability achieve significantly lower for PV-Tx than KC-Tx. Also allogeneic models (C57BL/6 BALB/C, n 104; C57BL/6, 77) primary KC-Tx affected survival. Histological evaluation livers showed presence features (embolism, thrombosis, focal areas liver necrosis) are absent subcapsular site. Finally, significant differences outcome observed between Th type 1 inflammatory-prone 2 mouse. Intraportal some more similar human should be used not only engraftment but also mechanisms immune suppression tolerance.