Biological Effects and Irradiation Dose Induced in Human Lymphocytes in Vitro by an Intracellular Radionuclide:99 m Tc

摘要: Since lymphocyte radiolabeling is widely used for in vitro and vivo studies may interfere with the properties of cells, purpose this work was to investigate effects /sup 99m/Tc labeling on morphology functions. Two ranges activity were used, 37 370 MBq, their compared those produced by a graduated external irradiation. Electron microscopy performed T cells studied using biological markers (E-Rosette formation, cell proliferation under lectin reactivity, specific cell-mediated cytolysis). After 370-MBq labeling, an intense cytotoxic effect observed morphology, drastically impeding T-lymphocyte Thirty-seven-megabecquerel sham procedure did not significantly affect The irradiation dose-response curve indicated clear radiation-induced inhibitory effect, especially over 10 Gy. This less than that obtained labeling. can then be attributed radiolesion due radiation dose which probably higher In fact, received lymphocytes during estimated as 70 Gy calculations, taking into account all emissions 99m/Tc.