The Secretion of Serum Protein and the Synthesis of Albumin and Total Protein in Regenerating Rat Liver

摘要: The regulation of albumin synthesis and serum protein secretion was studied in regenerating rat liver by measuring incorporation 14C-leucine. Albumin isolated to radiochemical purity, utilizing a method which eliminates the influence precursor pool changes on labeling. Changes size product were measured. following results obtained. 1. time period between intracaval injection 14Cleucine appearance radioactive blood ("secretion time") decreased from 15 min for normal animals minimum 10 at 48 hours after removal 70% liver. 2. At 14C-leucine, 3.5% total radioactivity found liver, whereas only 1.4% incorporated into albumin. 3. concentration 29.2 mg per ml rats 17.3 4 days partial hepatectomy. 4. half-life 2.66 2.13 animals, 5. intravascular 100 g, body wt, 57.8 partially hepatectomized operation. extravascular also hepatectomy 24 In contrast pool, increased again, reaching plateau 2 6. During regeneration, proportion leucine other amino acids did not change. Also, this differ significantly that 7. net rate changed slightly 20.1 day g 23.9 contrast, 576 1710