Effect of hormones and growth factors on the proliferation of adult cricket neural progenitor cells in vitro.

摘要: In the adult cricket brain, a cluster of neuroblasts produces new interneurons that integrate into mushroom body (MB), main associative structure for multisensory information insect brain. previous study we showed antagonist role two morphogenetic hormones, juvenile hormone (JH) and ecdysone, on regulation MB neurogenesis in vivo. order to examine whether these hormones act directly neural progenitor cells, developed an organotypic culture cortices. Cell proliferation was assessed by 5-bromo, 2′-deoxyuridine (BrdU) incorporation. We JH increased neuroblast (MBNb) proliferation, confirming mitogenic effect observed By contrast, ecdysone did not affect amount BrdU-labeled nuclei, suggesting inhibitory vivo probably proceeded from indirect pathway. then examined growth factors known stimulate stem cell/progenitor cell vertebrates. As shown calcium imaging, MBNb only expressed functional receptors insulin whereas mature responded IGF-I bFGF. Both (10 μg/mL) ng/mL) enhanced culture, although more pronounced. This abolished when inhibitor polyamine biosynthesis present medium, link between polyamines signaling bFGF (20–200 failed proliferation. Our results point conserved divergent mechanisms vertebrates invertebrates © 2003 Wiley Periodicals, Inc. J Neurobiol, 56: 387–397,