miR-204-5p Inhibits Proliferation and Invasion and Enhances Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating RAB22A

摘要: Purpose: miR-204-5p was found to be downregulated in colorectal cancer tissues our preliminary microarray analyses. However, the function of remains unknown. We therefore investigated role, mechanism, and clinical significance development progression. Experimental Design: measured expression determined its correlation with patient prognoses. Ectopic cells, xenografts, pulmonary metastasis models used evaluate effects on proliferation, migration, chemotherapy sensitivity. Luciferase assay Western blotting were performed validate potential targets after screening by a analysis computer-aided algorithms. Results: is frequently tissues, survival showed that downregulation associated poor repressed cell growth both vitro vivo . Moreover, restoring inhibited migration invasion promoted tumor sensitivity chemotherapy. Mechanistic investigations revealed RAB22A , member RAS oncogene family, direct functional target cancer. Furthermore, protein levels increased negatively time. Conclusions: Our results demonstrate for first time acts as suppressor through inhibiting reveal new prognostic factor Clin Cancer Res; 20(23); 6187–99. ©2014 AACR