Cathepsin D Degradable Dendrimer-MPEG-Histone 3-Enrofloxacin Conjugate Nanovehicle for Target Specific Bovine Mastitis Therapy

摘要: Mammary gland overexpresses cathepsin D aspartic enzymes during mastitis. Dendrimer (dend)-methoxy poly(ethylene glycol) (MPEG)-enrofloxacin (enro) conjugate nanoparticles were formulated for targeting mammary using and cleavable histone 3 peptide. Histone peptide was conjugated with the carboxylic acid end groups of a dendrimer, which then MPEG amine. The antibacterial agent, enrofloxacin dend-h3-MPEG conjugates. Dend-MPEG-enro conjugates without linkage also synthesized comparison. These converted into dialysis procedure. Particle size surface morphology developed measured photon correlation spectroscopy transmission electron microscope. In vitro drug release study dend-h3-MPEG-enro dend-MPEG-enro performed by bag diffusion technique over period 48 h. Conjugation within assessed UV–Vis spectrophotometer. mean (± SD) particle 69.4 ± 43 nm spherical circular in shape. had an absorption peak at 273.8 nm it confirmed successful conjugation enrofloxacin. Enrofloxacin released from via biodegradation upon exposure to D. From this study, can be suggested that could used deliver selectively exploiting expression