PDGF-BB inhibits intervertebral disc cell apoptosis in vitro

摘要: Degeneration of the intervertebral disc (IVD) results in deterioration spinal motion segment and can lead to debilitating back pain. Given established mitotic anti-apoptotic effects recombinant human platelet-derived growth factor-BB (rhPDGF-BB) a variety cell types we postulated that rhPDGF-BB might delay degeneration through inhibition apoptosis. To address this hypothesis, treated IVD cells isolated from five independent patients with monolayer 3D pellet cultures. The potential, proliferative capacity, morphology/pellet differentiation, gene expression PDGF-treated were evaluated via flow cytometry/immunohistochemistry, MTT assays, histology, quantitative RT-PCR, respectively. We found treatment significantly inhibited apoptosis, increased proliferation matrix production, maintained mRNA critical extracellular genes. This study suggests two possible mechanisms for anti-degenerative on cells. First, PDGF strongly apoptosis Second, acts as an anabolic agent, promoting maintenance phenotype culture, based molecular protein analysis. speculate may be used biologic target early degenerative disease future.