Dynamics of cold hardiness, supercooling and cryoprotectants in diapausing and non-diapausing pupae of the cabbage root fly, Delia radicum L.

摘要: Abstract The cabbage root fly, Delia radicum L., overwinters as a phanerocephalic pupa (PCP) in diapause state and its development may be temporarily stopped at the PCP stage even by high summer temperatures. Cold acclimation (5 days 5 °C) of non-diapausing resulted an increased cold hardiness (survival exposure to − 15 °C for 1 or 20 days) was associated with increase trehalose content (from 6.6 10.0 μg/mg wet weight). Non-diapausing pupae high-temperature quiescence had higher osmotic pressure haemolymph (623 mOsm) glucose (3.4 μg/mg) comparison normal (550 mOsm, 1.4 μg/mg). Diapausing start showed levels equal PCP. Their rapidly during first 10–30 attaining maximum between 30 50 followed obvious decrease. Osmotic from about 550 mOsm 860 day 80 then dropped slowly. Trehalose 4.7 11.2 declined too. Glucose transitional concentration abrupt drop until repeated toward termination. Myo-inositol level slowly showing plateau after days. Supercooling capacity similar irrespective physiological age pupa.