The Drosophila surface glia transcriptome: evolutionary conserved blood-brain barrier processes.

摘要: Abstract Central nervous system (CNS) function is dependent on the stringent regulation of metabolites, drugs, cells, and pathogens exposed to CNS space. Cellular blood-brain barrier (BBB) structures are highly specific checkpoints governing entry exit all small molecules from brain interstitial space, but precise mechanisms that regulate BBB not well understood. In addition, has long been a challenging obstacle pharmacologic treatment diseases; thus model systems can parse functions desirable. this study, we sought define transcriptome adult Drosophila melanogaster by isolating surface glia with FACS profiling their gene expression microarrays. By comparing these glia, neurons, whole brains, present catalog transcripts selectively enriched at BBB. We found fly show high many ABC SLC transporters, cell adhesion molecules, metabolic enzymes, signaling components xenobiotic metabolism pathways. Using sequence-based alignments, compare Murine transcriptomes discover shared chemoprotective molecule control pathways, affirming relevance invertebrate models for studying evolutionary conserved properties. The valuable vertebrate insect biologists alike as resource proteins underlying diffusion development maintenance, glial biology, drug transport tissue barriers.