Demonstration of B-cell maturation in X-linked immunodeficient mice by simultaneous three-colour immunofluorescence.

摘要: CBA/N mice carrying the X-linked immune deficiency gene (xid) have fewer splenic B cells than normal CBA and are unresponsive to a certain class of antigens. Studies B-cell surface-marker expression responsiveness led commonly accepted idea that in adult xid immature resemble young (1-3 week old) mice. That is, like animals, lack most numerous three IgM/IgD subpopulations (designated I Fig. 1a, b) present spleen. We now report, however, this picture is an oversimplification fact differ from those either or Using quantitative three-colour fluorescence-activated cell sorter (FACS) analyses, we compared correlated IgM, IgD newly discovered B-lymphocyte antigen (BLA-1) on show here (1) (as normals) BLA-1- whereas all animals BLA-1+; (2) major difference found between limited cells; (3) increased numbers BLA-1+ population III cells.